Application
Western blot analysis of MBD1 in HeLa cell lysate with anti-MBD1 mAb (Cat# ) at a dilution of 2 ug/ml (lane 1) and 1 ug/ml (lane 2). A protein band of approximate MW of 70 kDa is detected.| Product Name | MBD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [ 100B272.1 ] to Methyl-CpG-binding Domain 1 ( MBD1 )DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. |
| Immunogen | This antibody was generated by immunizing mice with a synthetic peptide of human MBD1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 100B272.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| This monoclonal antibody can be used for detection of MBD1, isoform 1, a 605 amino acid protein. In HeLa, a 70 kDa band is observed. Western blot analysis: 2 ug/ml.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586O0821 DMTase NY-CO-41 NY CO 41 NYCO41 |
|---|---|
| Target | MBD1 |
Application
Western blot analysis of MBD1 in HeLa cell lysate with anti-MBD1 mAb (Cat# ) at a dilution of 2 ug/ml (lane 1) and 1 ug/ml (lane 2). A protein band of approximate MW of 70 kDa is detected.| Positive Control | HeLaWCE |
|---|---|
| Application Notes | This monoclonal antibody can be used for detection of MBD1, isoform 1, a 605 amino acid protein. In HeLa, a 70 kDa band is observed. Western blot analysis: 2 ug/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH7.2 with 0.02% sodium azide as preservative. |
