Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:1000| Product Name | MBD1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MBD1 (methyl-CpG binding domain protein 1)DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MBD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CXXC3 antibody PCM1 antibody RFT antibody MBD1 antibody the regulator of fibroblast growth factor 2 (FGF-2) transcription antibody CXXC-type zinc finger protein 3 antibody methyl-CpG-binding domain protein 1 antibody methyl-CpG binding domain protei |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (5) |
| Cellular Localization | Nucleus speckle |
Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
