Application
Sample (30 ug of whole cell lysate) A: A431 B: JurKat C: Raji 10% SDS PAGE diluted at 1:1000| Product Name | MBD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MBD2 (methyl-CpG binding domain protein 2)DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MBD2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, ChickenMouse (98%), Rat (98%), Xenopus laevis (89%), Chicken (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586O0821 antibody DMTase antibody NY-CO-41 antibody MBD2 antibody methyl-CpG-binding domain protein 2 antibody demethylase antibody methyl-CpG binding domain protein 2 antibody |
|---|---|
| Target | MBD2 |
| Molecular Weight(MW) | 43 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A431 B: JurKat C: Raji 10% SDS PAGE diluted at 1:1000| Positive Control | A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
