Application
Western blot experiment using MBD3 Antibody at 1:90000 dilution to detect 100ng of 27kDa recombinant MBD3 protein antigen.Application
Sample (20 ug ) A: A431 nucleus 12% SDS PAGE diluted at 1:1000| Product Name | MBD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MBD3 (methyl-CpG binding domain protein 3)DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MBD3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (96%), Rat (96%), Xenopus laevis (93%), Chicken (96%), Rhesus Monkey (100%), Bovine (95%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MBD3 antibody methyl-CpG-binding domain protein 3 antibody methyl-CpG binding domain protein 3 antibody |
|---|---|
| Target | MBD3 |
| Molecular Weight(MW) | 33 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Western blot experiment using MBD3 Antibody at 1:90000 dilution to detect 100ng of 27kDa recombinant MBD3 protein antigen.
Application
Sample (20 ug ) A: A431 nucleus 12% SDS PAGE diluted at 1:1000| Positive Control | A431 nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
