Application
MBD4 antibody detects MBD4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MBD4 protein stained by MBD4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
MBD4 antibody detects MBD4 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of 3xFlag-human MBD4-transfected 293T cells 7.5 % SDS-PAGE MBD4 antibody dilution: 1:5000| Product Name | MBD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MBD4 (methyl-CpG binding domain protein 4)DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MBD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MED1 methyl-CpG binding domain protein 4 |
|---|---|
| Target | MBD4 |
| Molecular Weight(MW) | 66 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
MBD4 antibody detects MBD4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MBD4 protein stained by MBD4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
MBD4 antibody detects MBD4 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of 3xFlag-human MBD4-transfected 293T cells 7.5 % SDS-PAGE MBD4 antibody dilution: 1:5000| Positive Control | 3xFlag-human MBD4-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
