Application
HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MBD5 antibody diluted at 1:500.| Product Name | MBD5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MBD5 (methyl-CpG binding domain protein 5)This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause mental retardation autosomal dominant type 1. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MBD5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (96%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ11113 antibody FLJ30517 antibody KIAA1461 antibody MBD5 antibody MBD5 antibody MRD1 antibody methyl-CpG binding domain protein 5 antibody methyl-CpG-binding domain protein 5 antibody methyl-CpG-binding protein MBD5 antibody |
|---|---|
| Target | MBD5 |
| Molecular Weight(MW) | 160 kDa (note) |
| Cellular Localization | Nucleus |
Application
HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MBD5 antibody diluted at 1:500.| Positive Control | HepG2 , HepG2 nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
