Application
Sample (30 ug of whole cell lysate) A: K562 7.5% SDS PAGE diluted at 1:1000Application
MCAK antibody [N1N3] detects MCAK protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCAK protein stained by MCAK antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | MCAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCAK (kinesin family member 2C)The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCAK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (80%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KNSL6 antibody MCAK antibody KIF2C antibody kinesin-like 6 antibody kinesin-like protein KIF2C antibody kinesin-like protein 6 antibody mitotic centromere-associated kinesin antibody kinesin family member 2C antibody |
|---|---|
| Molecular Weight(MW) | 81 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton (By similarity) , Nucleus (By similarity) , Centromere |
Application
Sample (30 ug of whole cell lysate) A: K562 7.5% SDS PAGE diluted at 1:1000
Application
MCAK antibody [N1N3] detects MCAK protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCAK protein stained by MCAK antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
