Application
MCCC2 antibody detects MCCC2 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: MCCC2 protein stained by MCCC2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MCCC2 antibody at 1:250 dilution.Application
MCCC2 antibody detects MCCC2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE MCCC2 antibody dilution: 1:1000| Product Name | MCCC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCCC2 (methylcrotonoyl-CoA carboxylase 2 (beta))This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCCC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevisMouse (91%), Rat (91%), Zebrafish (82%), Xenopus laevis (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MCCB antibody MCCC2 antibody 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit antibody 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta antibody biotin carboxylase antibody MCCase subunit beta antibody 3-methylcrotonyl-CoA carbox |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
MCCC2 antibody detects MCCC2 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: MCCC2 protein stained by MCCC2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MCCC2 antibody at 1:250 dilution.
Application
MCCC2 antibody detects MCCC2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE MCCC2 antibody dilution: 1:1000| Positive Control | 293T , A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
