Application
mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. mCherry antibody labels mCherry-expressing cells dilution: 1:100.Application
mCherry antibody detects mCherry protein by western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
mCherry antibody detects mCherry (full length) protein by western blot analysis. A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12% SDS-PAGE mCherry antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis. Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min. Green: mCherry protein stained by mCherry antibody diluted at 1:500. Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.| Product Name | mCherry antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to mCherry |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-WIPWB |
| WB:1:100~1:200 IP:1:20~1:100 ICC/IF:1:20~1:100 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 27 kDa (note) |
|---|
Application
mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. mCherry antibody labels mCherry-expressing cells dilution: 1:100.
Application
mCherry antibody detects mCherry protein by western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
mCherry antibody detects mCherry (full length) protein by western blot analysis. A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12% SDS-PAGE mCherry antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis. Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min. Green: mCherry protein stained by mCherry antibody diluted at 1:500. Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.| Positive Control | human PLK4-mCherry-transfected 293T |
|---|---|
| Application Notes | WB:1:100~1:200 IP:1:20~1:100 ICC/IF:1:20~1:100 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
