Application
Flow cytometric analysis of Hela cells, using anti-MCL1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-MCL1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded lung tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells transfected with either pCMV6-ENTRY MCL1Application
Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MCL1Application
Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded bladder tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.| Product Name | MCL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2E11] to MCL1Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Immunogen | Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2E11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MCL1L MGC104264 MGC1839 MCL1 TM MCL1S EAT |
|---|---|
| Target | MCL1 |
| Tissue Specificity | is specific for human MCL1 (NP_068779). |
| Cellular Localization | Cytoplasmic, associated with mitochondria. |
Application
Flow cytometric analysis of Hela cells, using anti-MCL1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-MCL1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded lung tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells transfected with either pCMV6-ENTRY MCL1
Application
Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MCL1
Application
Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded bladder tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
