Application
WB analysis of Jurkat cells using MCM10 antibody at 0.2-1μg/ml.| Product Name | MCM10 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM10The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human MCM10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 77%; Dog: 86%; Horse: 93%; Human: 100%; Pig: 77%; Rabbit: 86% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CNA43 DNA43 PRO2249 MCM10 |
|---|---|
| Target | MCM10 |
| Molecular Weight(MW) | 98 KDa (note) |
Application
WB analysis of Jurkat cells using MCM10 antibody at 0.2-1μg/ml.| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
