Application
ELISA analysis of MCM2 antibody [2B3] using control (100 ng) and antigen (10 ng, 50 ng, 100 ng).Application
WB analysis of PC-12 (1), Cos7 (2), NIH3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysates using MCM2 antibody [2B3].Application
FACS analysis of HeLa cells using MCM2 antibody [2B3] (blue) and a negative control (red).Application
IHC-P analysis of colon cancer tissues using MCM2 antibody [2B3].| Product Name | MCM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [2B3] to MCM2The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] |
| Immunogen | The immunogen corresponding to human MCM2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2B3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CDCL1 D3S3194 BM28 MITOTIN CCNL1 CDC19 |
|---|---|
| Target | MCM2 |
| Molecular Weight(MW) | 125 kDa (note) |
Application
ELISA analysis of MCM2 antibody [2B3] using control (100 ng) and antigen (10 ng, 50 ng, 100 ng).
Application
WB analysis of PC-12 (1), Cos7 (2), NIH3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysates using MCM2 antibody [2B3].
Application
FACS analysis of HeLa cells using MCM2 antibody [2B3] (blue) and a negative control (red).
Application
IHC-P analysis of colon cancer tissues using MCM2 antibody [2B3].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
