Application
MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 40 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000| Product Name | MCM2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM2 (minichromosome maintenance complex component 2)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (88%), Rat (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BM28 antibody CCNL1 antibody CDCL1 antibody D3S3194 antibody KIAA0030 antibody MGC10606 antibody MITOTIN antibody cdc19 antibody MCM2 antibody cell devision cycle-like 1 antibody DNA replication licensing factor MCM2 antibody nuclear protein BM2 |
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| Molecular Weight(MW) | 102 kDa (note) |
Application
MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 40 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
