Application
MCM3 antibody immunoprecipitates MCM3 protein in IP experiments. IP samples: HepG2 whole cell extract A. 40 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM3 protein by 4 μg MCM3 antibody 7.5 % SDS-PAGE The immunoprecipitated MCM3 protein was detected by MCM3 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.Application
MCM3 antibody detects MCM3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM3 protein stained by MCM3 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.Application
MCM3 antibody detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MCM3 antibody diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | MCM3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MCM3 (minichromosome maintenance complex component 3)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCM3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (94%), Zebrafish (80%), Xenopus laevis (84%), Chicken (86%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HCC5 antibody MGC1157 antibody P1-MCM3 antibody P1.h antibody RLFB antibody MCM3 antibody RLF subunit beta antibody MCM3 minichromosome maintenance deficient 3 antibody minichromosome maintenance deficient 3 antibody DNA replication factor MCM3 an |
|---|---|
| Target | MCM3 |
| Molecular Weight(MW) | 91 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
MCM3 antibody immunoprecipitates MCM3 protein in IP experiments. IP samples: HepG2 whole cell extract A. 40 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM3 protein by 4 μg MCM3 antibody 7.5 % SDS-PAGE The immunoprecipitated MCM3 protein was detected by MCM3 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
Application
MCM3 antibody detects MCM3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM3 protein stained by MCM3 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Application
MCM3 antibody detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MCM3 antibody diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
