Application
MCM4 antibody detects MCM4 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE MCM4 antibody dilution: 1:5000Application
MCM4 antibody immunoprecipitates MCM4 protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody 7.5 % SDS-PAGE The immunoprecipitated MCM4 protein was detected by MCM4 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM3 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using MCM4 antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM4 antibody at 1:500 dilution.| Product Name | MCM4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM4 (minichromosome maintenance complex component 4)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineRat (95%), Zebrafish (88%), Xenopus laevis (89%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC21 antibody CDC54 antibody MGC33310 antibody P1-CDC21 antibody hCdc21 antibody MCM4 antibody CDC21 homolog antibody homolog of S. pombe cell devision cycle 21 antibody MCM4 minichromosome maintenance deficient 4 antibody minichromosome maintena |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
MCM4 antibody detects MCM4 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE MCM4 antibody dilution: 1:5000
Application
MCM4 antibody immunoprecipitates MCM4 protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody 7.5 % SDS-PAGE The immunoprecipitated MCM4 protein was detected by MCM4 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM3 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using MCM4 antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM4 antibody at 1:500 dilution.| Positive Control | Jurkat , Raji , NCI-H929 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
