Application
MCM5 antibody detects MCM5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM5 protein stained by MCM5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using MCM5 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE MCM5 antibody diluted at 1:1000| Product Name | MCM5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM5 (minichromosome maintenance complex component 5)The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MCM5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Chicken, BovineMouse (95%), Xenopus laevis (88%), Chicken (86%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC46 antibody MGC5315 antibody P1-CDC46 antibody MCM5 antibody MCM5 minichromosome maintenance deficient 5 cell division cycle 46 antibody minichromosome maintenance deficient 5 (cell division cycle 46) antibody DNA replication licensing factor MCM |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
MCM5 antibody detects MCM5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM5 protein stained by MCM5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using MCM5 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE MCM5 antibody diluted at 1:1000| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
