Application
WB analysis of extracts from various cells using MCM7 antibody.Application
IHC-P analysis of human breast cancer using MCM7 antibody at a dilution of 1:200.Application
IHC-P analysis of human esophageal cancer using MCM7 antibody at a dilution of 1:200.Application
IHC-P analysis of human rectal cancer using MCM7 antibody at a dilution of 1:200.Application
IHC-P analysis of rat brain using MCM7 antibody at a dilution of 1:200.Application
ICC/IF analysis of HeLa cells using MCM7 antibody (green) and DAPI (blue).| Product Name | MCM7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCM7The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human MCM7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | minichromosome maintenance complex component 7 P1.1-MCM3 P1CDC47 PNAS146 P85MCM CDC47 MCM2 |
|---|---|
| Target | MCM7 |
| Molecular Weight(MW) | 81 kDa (note) |
Application
WB analysis of extracts from various cells using MCM7 antibody.
Application
IHC-P analysis of human breast cancer using MCM7 antibody at a dilution of 1:200.
Application
IHC-P analysis of human esophageal cancer using MCM7 antibody at a dilution of 1:200.
Application
IHC-P analysis of human rectal cancer using MCM7 antibody at a dilution of 1:200.
Application
IHC-P analysis of rat brain using MCM7 antibody at a dilution of 1:200.
Application
ICC/IF analysis of HeLa cells using MCM7 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
