Application
MCM2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of MCM2 Antibody in HL-60 cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | MCM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.1) Saade, E., et al. Proteomics 9(21):4934-4943(2009) |
| Immunogen | This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCM2 |
|---|---|
| Alternative Names | DNA replication licensing factor MCM2 Minichromosome maintenance protein 2 homolog Nuclear protein BM28 MCM2 BM28 CCNL1 CDCL1 KIAA0030 |
| Molecular Weight(MW) | 102 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
MCM2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of MCM2 Antibody in HL-60 cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
