Application
Anti-TRPML2 - Western blot analysis of MCF-7 (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates: 1-3. Anti-TRPML2 antibody, (1:200). 4-6. Anti-TRPML2 antibody, preincubated with the control peptide antigen.Application
Anti-TRPML2 - Expression of TRPML2 in rat heart Immunohistochemical staining of rat heart paraffin-embedded sections using Anti-TRPML2 antibody, followed by AlexaFluor-555 secondary antibody. A. TRPML2 labeling appears specifically in the cardiac muscle fibers and in a lesser intensity in the endothelium. It does not appear in the smooth muscle fibers of blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.Application
Anti-TRPML2 - Expression of TRPML2 in mouse muscle myoblast (C2C12) cell line Immunocytochemical staining of TRPML2 in C2C12 cells. A. Cells were stained with Anti-TRPML2 antibody, (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI is used as the counter staining (blue). C. Merged images of A and B.| Product Name | Mcoln2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Mcoln2 |
| Immunogen | Peptide (C)RLDFYRLVQVDISFALK, corresponding to amino acid residues 212-228 of mouse TRPML2 (Accession Q8K595). Intracellular, N-terminus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C86638 AI549968 TRPML2 3300002C04Rik mucolipin 2 |
|---|---|
| Target | Mcoln2 |
Application
Anti-TRPML2 - Western blot analysis of MCF-7 (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates: 1-3. Anti-TRPML2 antibody, (1:200). 4-6. Anti-TRPML2 antibody, preincubated with the control peptide antigen.
Application
Anti-TRPML2 - Expression of TRPML2 in rat heart Immunohistochemical staining of rat heart paraffin-embedded sections using Anti-TRPML2 antibody, followed by AlexaFluor-555 secondary antibody. A. TRPML2 labeling appears specifically in the cardiac muscle fibers and in a lesser intensity in the endothelium. It does not appear in the smooth muscle fibers of blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
Application
Anti-TRPML2 - Expression of TRPML2 in mouse muscle myoblast (C2C12) cell line Immunocytochemical staining of TRPML2 in C2C12 cells. A. Cells were stained with Anti-TRPML2 antibody, (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI is used as the counter staining (blue). C. Merged images of A and B.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
