Application
Anti-TRPML3 - Western blot analysis of rat brain (lanes 1 and 4), rat kidney (lanes 2 and 5) and rat pancreas (lanes 3 and 6): 1, 2, 3. Anti-TRPML3 antibody, (1:200). 4, 5, 6. Anti-TRPML3 antibody, preincubated with the control peptide antigen.Application
Anti-TRPML3 - Expression of TRPML3 in mouse brain stem Immunohistochemical staining of TRPML3 in mouse brain stem using Anti-TRPML3 antibody. A. TRPML3 (green) appears in neurons (arrows) in the area of the dorsal cochlear nucleus. B. Staining of the same section with mouse anti-parvalbumin (red) reveals that the TRPML3 appears in a subset of neurons in this nucleus. DAPI is used as the counterstain (blue).| Product Name | MCOLN3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCOLN3 |
| Immunogen | Peptide CKDLPNSGKYRLEDD, corresponding to amino acid residues 528-542 of mouse TRPML3 (Accession Q8R4F0). Intracellular, C-terminus (cytoplasmic). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Mcoln3 mucolipin 3 TRPML3 6720490O21Rik Va |
|---|---|
| Target | MCOLN3 |
Application
Anti-TRPML3 - Western blot analysis of rat brain (lanes 1 and 4), rat kidney (lanes 2 and 5) and rat pancreas (lanes 3 and 6): 1, 2, 3. Anti-TRPML3 antibody, (1:200). 4, 5, 6. Anti-TRPML3 antibody, preincubated with the control peptide antigen.
Application
Anti-TRPML3 - Expression of TRPML3 in mouse brain stem Immunohistochemical staining of TRPML3 in mouse brain stem using Anti-TRPML3 antibody. A. TRPML3 (green) appears in neurons (arrows) in the area of the dorsal cochlear nucleus. B. Staining of the same section with mouse anti-parvalbumin (red) reveals that the TRPML3 appears in a subset of neurons in this nucleus. DAPI is used as the counterstain (blue).| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
