| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [24822.11] to MCP1Monocyte chemotactic protein (MCP1) is also known as monocyte chemotactic and activating factor (MCAF). It is the product of the human JE gene. The precursor form of MCP1 consists of 99 amino acids with a signal peptide sequence consisting of 23 amino-terminal acids. The mature form of MCP1 has 4 cysteine residues. The first two cysteine residues are in an adjacent position C-C, which characterizes MCP1 as a member of the chemokine beta subfamily. rhMCP1 is a non-glycosylated protein consisting of 76 amino acids with a molecular weight of 8.7 kDa. MCP1 mRNA expression can be induced in monocytes/macrophages, B lymphocytes, endothelial cells and astrocytoma cells by LPS. IL1 will induce production of MCP1 in fibroblasts, keratinocytes, hepatoma cells and Type II pneumocytes. In vitro, MCP1 will act on monocytes to initiate chemotaxis, induce superoxide anion release, induce the release of lysosomal enzymes, and augment cytostatic activity. In vivo, MCP1 will induce macrophage infiltration. The MCP1 gene contains potential binding sites for several transcription factors, including AP1, AP2, NFkB, and NFIL6. |
| Immunogen | Recombinant human MCP-1 (rhMCP-1), expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 24822.11 |
| Host Species | Mouse |
| Tested Applications | Neut |
| Neut: Use at a concentration of 0.1 μg/ml. Human MCP1 is tested for its ability to neutralize the chemoattractant activity of rhMCP1 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP1 that was present at a concentration just high enough to elicit a maximum response. In this bioassay, rhMCP1 was preincubated with various dilutions of the antibody for 1 hour at 37 °C in a 96-well microtiter plate. Then, the MCP1 antibody solution containing rhMCP1 at a final concentration of 0.1 μg/ml was transferred to the lower compartment of a 96-well chemotaxis chamber. The chemotaxis chamber was assembled using a PVP-free polycarbonate filter and the monocytes were added to the top chamber. The chamber was incubated for 90 minutes at 37 °C in a 5% CO2 humidified incubator. The chamber was disassembled and the filter fixed and stained. The optical density of the filter, which is proportional to the number of cells that migrated across the filter, was read with a microtiter plate reader set at 540 nm. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | GDCF-2 HC11 HSMCR30 MCAF MCP-1 MCP1 MGC9434 SCYA2 SMC-CF MCP 1 GDCF 2 SMC CF GDCF2 SMCCF |
|---|---|
| Target | MCP1 |
| Cellular Localization | Secreted |
| Application Notes | Neut: Use at a concentration of 0.1 μg/ml. Human MCP1 is tested for its ability to neutralize the chemoattractant activity of rhMCP1 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP1 that was present at a concentration just high enough to elicit a maximum response. In this bioassay, rhMCP1 was preincubated with various dilutions of the antibody for 1 hour at 37 °C in a 96-well microtiter plate. Then, the MCP1 antibody solution containing rhMCP1 at a final concentration of 0.1 μg/ml was transferred to the lower compartment of a 96-well chemotaxis chamber. The chemotaxis chamber was assembled using a PVP-free polycarbonate filter and the monocytes were added to the top chamber. The chamber was incubated for 90 minutes at 37 °C in a 5% CO2 humidified incubator. The chamber was disassembled and the filter fixed and stained. The optical density of the filter, which is proportional to the number of cells that migrated across the filter, was read with a microtiter plate reader set at 540 nm. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS |
