Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Immunofluorescence analysis of HepG2 cells using CCL2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Flow cytometric analysis of A549 cells using CCL2 mouse mAb (green) and negative control (red).Application
Western blot analysis using CCL2 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.Application
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 mouse mAb with DAB staining.| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [2D8] to CCL2This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human CCL2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | SMC-CF SCYA2 CCL2 MCP-1 MCAF HC11 HSMCR30 GDCF-2 MCP1 |
|---|---|
| Target | CCL2 |
| Molecular Weight(MW) | 11 kDa (note) |
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Immunofluorescence analysis of HepG2 cells using CCL2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Flow cytometric analysis of A549 cells using CCL2 mouse mAb (green) and negative control (red).
Application
Western blot analysis using CCL2 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 mouse mAb with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
