| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [S101] to MCP1This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | S101 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at an assay dependent dilution. Neut: Use at a concentration of 0.5 μg/ml. WB: Use at a concentration of 0.1 - 5 μg/ml. At a concentration of 0.1 - 1.0 μg/mL the antibody will allow visualization of 0.1 μg/lane of human MCP1. Concentration of 5.0 μg/mL will allow visualization of 50 μg/lane of rabbit MCP1. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRabbit |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Small inducible cytokine subfamily A Cys Cys member2 Monocyte chemoattractant protein 1 Monocyte secretory protein JE CCL2 Monocyte chemoattractant protein1 MCP1 Small inducible cytokine subfamily A Cys Cys member 2 Monocyte chemotactic and activati |
|---|---|
| Target | MCP1 |
| Tissue Specificity | reacts with natural and recombinant human MCP1. It does not react with human interleukin 8 (IL8) and other human cytokines tested such as interleukin 1 beta (IL1 beta), serum amyloid A (SAA) and epidermal growth factor (EGF). |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at an assay dependent dilution. Neut: Use at a concentration of 0.5 μg/ml. WB: Use at a concentration of 0.1 - 5 μg/ml. At a concentration of 0.1 - 1.0 μg/mL the antibody will allow visualization of 0.1 μg/lane of human MCP1. Concentration of 5.0 μg/mL will allow visualization of 50 μg/lane of rabbit MCP1. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 0.01M PBS. pH 7.2 |
