| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [S14] to MCP1This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | S14 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| For ELISA: Use at an assay dependent dilution. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated or biotin-conjugated). Suggested Capture Coating Dose: 0.3μg/well Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600 pg/mL. For Neut: Use at a concentration of 0.5 μg/ml. Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinant human MCP-1 in blindwell chambers showed that the antibodies were particularly effective at blocking MCP-1 activity at a concentration of 0.5μg/mL. It was also found that S-14 could inhibit the function of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. For WB: Use at a concentration of 0.1-1.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Small inducible cytokine subfamily A Cys Cys member2 Monocyte chemoattractant protein 1 Monocyte secretory protein JE CCL2 Monocyte chemoattractant protein1 MCP1 Small inducible cytokine subfamily A Cys Cys member 2 Monocyte chemotactic and activati |
|---|---|
| Target | MCP1 |
| Tissue Specificity | This monoclonal antibody reacts with natural and recombinant human MCP-1. Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1? (IL-1?), serum amyloid A (SAA) and epidermal growth factor (EGF). |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
| Application Notes | For ELISA: Use at an assay dependent dilution. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated or biotin-conjugated). Suggested Capture Coating Dose: 0.3μg/well Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600 pg/mL. For Neut: Use at a concentration of 0.5 μg/ml. Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinant human MCP-1 in blindwell chambers showed that the antibodies were particularly effective at blocking MCP-1 activity at a concentration of 0.5μg/mL. It was also found that S-14 could inhibit the function of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. For WB: Use at a concentration of 0.1-1.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH7.2 |
