| Product Name | M-CSF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to M-CSFMacrophage Colony Stimulating Factor (M-CSF) is produced by various cell types including fibroblasts, macrophages and endothelial cells. This colony stimulation factor is involved in both the development and function of monocytes/macrophages. |
| Immunogen | Highly pure (>98%) recombinant hM-CSF (human M-CSF) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 μg/ml of this antibody is required. ELISA:To detect hM-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSF1 Macrophage Colony Stimulating Factor 1 |
|---|---|
| Target | M-CSF |
| Cellular Localization | Secreted |
| Application Notes | Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 μg/ml of this antibody is required. ELISA:To detect hM-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS, pH 7.4, no preservative, sterile filtered |
