Application
MCT1 antibody [GT14612] detects MCT1 protein at weak membrane and strong cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. MCT1 antibody [GT14612] dilution: 1:500.Application
MCT1 antibody detects MCT1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
MCT1 antibody [GT14612] detects MCT1 protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCT1 protein stained by MCT1 antibody [GT14612] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | MCT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT14612] to MCT1 (solute carrier family 16, member 1 (monocarboxylic acid transporter 1))The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MCT1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT14612 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | solute carrier family 16 member 1 (monocarboxylic acid transporter 1) HHF7 SLC16A1 MCT |
|---|---|
| Target | MCT1 |
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
MCT1 antibody [GT14612] detects MCT1 protein at weak membrane and strong cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. MCT1 antibody [GT14612] dilution: 1:500.
Application
MCT1 antibody detects MCT1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
MCT1 antibody [GT14612] detects MCT1 protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCT1 protein stained by MCT1 antibody [GT14612] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
Data sheet for OM262744