Application
Immunohistochemical staining of rat spleen cells using MD-2 antibody at 2 ug/ml.Application
Western blot analysis of MD-2 in mouse spleen cell lysate with MD-2 antibody at 1 ug/ml.Application
Immunofluorescence of MD-2 in Rat Spleen cells with MD-2 antibody at 10 ug/mL.| Product Name | MD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MD-2MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS (5,6). The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4 (7). |
| Immunogen | Human MD-2 (Intermediate Domain) Peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MD2 MD-2 MD 2 |
|---|---|
| Target | MD-2 |
| Tissue Specificity | This antibody is specific for MD-2 Internal |
Application
Immunohistochemical staining of rat spleen cells using MD-2 antibody at 2 ug/ml.
Application
Western blot analysis of MD-2 in mouse spleen cell lysate with MD-2 antibody at 1 ug/ml.
Application
Immunofluorescence of MD-2 in Rat Spleen cells with MD-2 antibody at 10 ug/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
