Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 5% SDS PAGE diluted at 1:1000| Product Name | MDC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDC1 (mediator of DNA-damage checkpoint 1)The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (96%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp781A0122 antibody KIAA0170 antibody MGC166888 antibody NFBD1 antibody MDC1 antibody homologue to Drosophila photoreceptor protein calphotin antibody nuclear factor with BRCT domains 1 antibody mediator of DNA damage checkpoint 1 antibody media |
|---|---|
| Molecular Weight(MW) | 227 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 5% SDS PAGE diluted at 1:1000| Positive Control | HeLa nucleus |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
