Application
ME3 antibody detects ME3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with ME3 antibody at a dilution of 1:500.| Product Name | ME3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ME3 (malic enzyme 3, NADP(+)-dependent, mitochondrial)Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ME3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Pig, BovineHuman (100%), Pig (96%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NADP-ME malic enzyme 3 NADP(+)-dependent mitochondrial |
|---|---|
| Target | ME3 |
| Molecular Weight(MW) | 67 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
ME3 antibody detects ME3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with ME3 antibody at a dilution of 1:500.| Positive Control | mouse hippocampus |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
