Application
MED10 antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
293T whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 antibody diluted at 1:500.| Product Name | MED10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED10 (mediator complex subunit 10)MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MED10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (85%), Xenopus laevis (92%), Rhesus Monkey (98%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TRG20 NUT2 L6 mediator complex subunit 10 |
|---|---|
| Target | MED10 |
| Molecular Weight(MW) | 16 kDa (note) |
| Cellular Localization | Nucleus |
Application
MED10 antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
293T whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 antibody diluted at 1:500.| Positive Control | 293T , 293T nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
