Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MED19 antibody diluted at 1:1000.| Product Name | MED19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED19 (mediator complex subunit 19)MED19 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MED19. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (95%), Rat (95%), Xenopus laevis (82%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LCMR1 mediator complex subunit 19 DT2P1G7 |
|---|---|
| Target | MED19 |
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MED19 antibody diluted at 1:1000.| Positive Control | Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
