Application
Human Uterus (formalin-fixed, paraffin-embedded) stained with MED23 antibody at 1:100 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | MED23 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MED23Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the preinitiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling. |
| Immunogen | Synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB (1:1000), IP (1:100 - 1:200), ELISA, IHC-P (1:100): |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | 130kDa 3000002A17Rik Crsp3 ESTM7 Sur2 X83317 mKIAA1216 |
|---|---|
| Tissue Specificity | Recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130). Cross-reactivity occurs with human DRIP-130. Reactivity with DRIP-130 isolated from other species is unknown. |
Application
Human Uterus (formalin-fixed, paraffin-embedded) stained with MED23 antibody at 1:100 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1:1000), IP (1:100 - 1:200), ELISA, IHC-P (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Long term: -20°C; Short term: +4°C |
| Storage Buffer | PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. |
