Application
MED27 antibody detects MED27 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MED27 antibody diluted by 1:1000.| Product Name | MED27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED27 (mediator complex subunit 27)The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MED27. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (96%), Rat (97%), Zebrafish (87%), Xenopus laevis (87%), Pig (97%), Chicken (88%), Rhesus Monkey (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TRAP37 CRAP34 CRSP34 mediator complex subunit 27 CRSP8 |
|---|---|
| Target | MED27 |
| Molecular Weight(MW) | 35 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
MED27 antibody detects MED27 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MED27 antibody diluted by 1:1000.| Positive Control | A549 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
