Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using MED4 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000| Product Name | MED4 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED4 (mediator of RNA polymerase II transcription subunit 4)The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II (see MIM 180660)-transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (summary by Sato et al., 2004 [PubMed 15175163]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MED4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARC36 antibody DRIP36 antibody FLJ10956 antibody HSPC126 antibody TRAP36 antibody VDRIP antibody MED4 antibody activator-recruited cofactor 36 kDa component antibody vitamin D3 receptor-interacting protein complex 36 kDa component antibody mediato |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using MED4 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
