Application
Western blot analysis of MEK2 in 15 ugs of 293 cell lysate at 1 ug/ml.| Product Name | MEK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MEK2Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 1 - 3 μg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Gene Synonyms | FLJ26075 MAPKK2 MEK2 MKK2 PRKMK2 |
|---|---|
| Target | MEK2 |
Application
Western blot analysis of MEK2 in 15 ugs of 293 cell lysate at 1 ug/ml.| Positive Control | 293 whole cell lysate. |
|---|---|
| Application Notes | WB: Use at a concentration of 1 - 3 μg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide, 0.2% GelatinConstituents: PBS |
