Application
The anti-MAP2K2 Pab is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.Application
Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
MAP2K2 Antibody (A278) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow).| Product Name | MEK2 (MAP2K2) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK2 MKK2 PRKMK2 |
|---|---|
| Alternative Names | MAP2K2 MEK2 MKK2 PRKMK2 Dual specificity mitogen-activated protein kinase kinase 2 ERK activator kinase 2 MAPK/ERK kinase 2 |
| Molecular Weight(MW) | 44424 Da |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity) |
| Tissue Specificity | This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271~300 amino acids from the center region of human MAP2K2. |
| Entrez Gene | 5605 |
|---|
Application
The anti-MAP2K2 Pab is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.
Application
Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
MAP2K2 Antibody (A278) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
