Application
Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.Application
Figure 2:Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 3: Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).| Product Name | MAP2K2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MEK2; MKK2; MAPKK2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F5 |
| Host Species | Mouse |
| Tested Applications | WBIFFC |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK2 MKK2 PRKMK2 |
|---|---|
| Alternative Names | MEK2 MKK2 MAPKK2 |
| Molecular Weight(MW) | 44424 Da |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity) |
| Tissue Specificity | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
| Entrez Gene | 5605 |
|---|---|
| Nucleotide Accession | NP_109587.1 |
Application
Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.
Application
Figure 2:Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 3: Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
