Application
Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.Application
Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MAP2K2 Antibody (S222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK2 MKK2 PRKMK2 |
|---|---|
| Alternative Names | MAP2K2 MEK2 MKK2 PRKMK2 Dual specificity mitogen-activated protein kinase kinase 2 ERK activator kinase 2 MAPK/ERK kinase 2 |
| Molecular Weight(MW) | 44424 Da |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity) |
| Tissue Specificity | This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S222 of human MAP2K2. |
| Entrez Gene | 5605 |
|---|
Application
Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
