Application
MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MEK3 antibody [N1C3] diluted at 1:500.Application
MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MEK3 antibody [N1C3] diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MEK3 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using MEK3 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: Raji 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:500| Product Name | MEK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK3 (mitogen-activated protein kinase kinase 3)The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (97%), Xenopus laevis (80%), Chicken (90%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK3 antibody, MEK3 antibody, MKK3 antibody, PRKMK3 antibody, MAP2K3 antibody, MAPK/ERK kinase 3 antibody, MEK 3 antibody, SAPK kinase 2 antibody, MAP kinase kinase 3 antibody, MAPKK 3 antibody, dual specificity mitogen-activated protein kinase kinase 3 |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
Application
MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MEK3 antibody [N1C3] diluted at 1:500.
Application
MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MEK3 antibody [N1C3] diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MEK3 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MEK3 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: Raji 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
