Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MEK5 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:2000| Product Name | MEK5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK5 (mitogen-activated protein kinase kinase 5)The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEK5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (98%), Rat (98%), Chicken (91%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HsT17454 antibody, MAPKK5 antibody, MEK5 antibody, PRKMK5 antibody, MAP2K5 antibody, MEK 5 antibody, MAPK/ERK kinase 5 antibody, MAPKK 5 antibody, dual specificity mitogen-activated protein kinase kinase 5 antibody, MAP kinase kinase 5 antibody, MAP kinase |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MEK5 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:2000| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
