Application
Extracts prepared from background extracts with GST-tagged fusion protein expressing MEK7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight| Product Name | MEK7 (phospho Ser271/Thr275) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MEK7 ( phospho S271 + T275 )Mitogen Activated Protein Kinase Kinase 7 (MEK 7 or MKK 7) is a 38 kDa member of a tyrosine/threonine protein kinase family that activates the c Jun NH2-terminal kinases (JNK), which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK 7 by MEKK 1 on serine 271 and threonine 275 in the catalytic domain activates the protein and enables MEK 7 to phosphorylate JNK. |
| Immunogen | Synthetic peptide (Human) derived from a region of human MEK 7 that contains serine 271 and threonine 275. The sequence is conserved in mouse. |
| Modification | p-Ser271/Thr275 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a concentration of 0.1-1.0 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Jnkk2, MAPKK7, MKK7, PRKMK7, MEK7 (phospho S271/T275), MEK7 (phospho Ser271/Thr275), MEK7 (pS271/pT275), MEK7 (pSer271/pThr275), MEK7 phospho S271/T275, MEK7 phospho Ser271/Thr275, Phospho MEK7 (S271/T275), Phospho MEK7 (Ser271/Thr275), Phospho MEK7 (pS27 |
|---|---|
| Target | MEK7 |
| Tissue Specificity | This antibody does not react with other MEK isoforms. |
Application
Extracts prepared from background extracts with GST-tagged fusion protein expressing MEK7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight| Positive Control | Fusion protein expressing MEK 7 |
|---|---|
| Application Notes | For WB: Use at a concentration of 0.1-1.0 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3 |
