Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using MEKK3 antibody at 1:500 dilution.Application
MEKK3 antibody [N3C2], Internal detects MEKK3 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of 3xFlag-human MAP3K3-transfected 293T cells 7.5 % SDS-PAGE MEKK3 antibody [N3C2], Internal dilution: 1:5000| Product Name | MEKK3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEKK3 (mitogen-activated protein kinase kinase kinase 3)This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEKK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (97%), Rat (97%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAPKKK3 antibody, MEKK3 antibody, MAP3K3 antibody, MAP/ERK kinase kinase 3 antibody, MEKK 3 antibody, mitogen-activated protein kinase kinase kinase 3 antibody, MEK kinase 3 antibody, MAPK/ERK kinase kinase 3 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (3) |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using MEKK3 antibody at 1:500 dilution.
Application
MEKK3 antibody [N3C2], Internal detects MEKK3 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of 3xFlag-human MAP3K3-transfected 293T cells 7.5 % SDS-PAGE MEKK3 antibody [N3C2], Internal dilution: 1:5000| Positive Control | 3xFlag-human MAP3K3-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
