Application
Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody and the same antibody preincubated with blocking peptide| Product Name | Melanoma-associated antigen D1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Melanoma-associated antigen D1Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation. |
| Immunogen | Peptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | melanoma-associated antigen D1, neurotrophin receptor-interacting MAGE homolog, MAGE-D1 antigen, 2810433C11Rik, 5430405L04Rik, DXBwg1492e, Dlixin, Dlxin-1, Dlxin1, FLJ00163, NRAGE, mFLJ00163, Maged1, melanoma antigen, family D, 1 |
|---|---|
| Target | Melanoma-associated antigen D1 |
| Molecular Weight(MW) | 95 (note) |
Application
Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody and the same antibody preincubated with blocking peptide| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
