Rabbit Polyclonal antibody to MELKPhosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Immunogen
Peptide sequence around aa.626~630 (I-R-R-Q-R) derived from Human MELK.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
HPK38, KIAA0175, MELK, pEg3 kinase, protein kinase Eg3, maternal embryonic leucine zipper kinase, hMELK, tyrosine-protein kinase MELK, protein kinase PK38
Target
MELK
Molecular Weight(MW)
74 (note)
Application
Application
Western blot analysis of extract from Rat brain tissue using MELK Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.