Application
Sample (30 µg of whole cell lysate) A: HeLa B: HCT116 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MELK antibody detects MELK protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MELK antibody diluted at 1:500.Application
MELK antibody detects MELK protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MELK antibody diluted at 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MELK antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | MELK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MELK (maternal embryonic leucine zipper kinase) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MELK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, BovineRat (96%), Xenopus laevis (80%), Chicken (80%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HPK38 antibody, KIAA0175 antibody, MELK antibody, pEg3 kinase antibody, protein kinase Eg3 antibody, maternal embryonic leucine zipper kinase antibody, hMELK antibody, tyrosine-protein kinase MELK antibody, protein kinase PK38 antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HCT116 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MELK antibody detects MELK protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MELK antibody diluted at 1:500.
Application
MELK antibody detects MELK protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MELK antibody diluted at 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MELK antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
