Application
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.Application
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.| Product Name | Mer2 (phospho Ser30) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Mer2 (phospho Ser30)Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog.This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with the replication fork. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein. |
| Modification | p-Ser30 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | S.cerevisiae |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | REC107 MER2 |
|---|---|
| Target | Mer2 Ps30 |
| Cellular Localization | Localizes to chromosomes. |
Application
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.
Application
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
