Application
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.| Product Name | Mer2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Mer2Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases.Moreover, there are hints that the |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | S.cerevisiae |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MER2 REC107 |
|---|---|
| Target | Mer2 |
| Cellular Localization | Localizes to chromosomes. |
Application
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
