Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Neurofibromin 2 antibody at 1:500 dilution.Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Neurofibromin 2 antibody at 1:500 dilution.| Product Name | Merlin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Neurofibromin 2 (neurofibromin 2 (merlin))This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Neurofibromin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus MonkeyXenopus laevis (92%), Chicken (95%), Rhesus Monkey (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACN antibody BANF antibody SCH antibody NF2 antibody moesin-ezrin-radixin-like protein antibody moesin-ezrin-radixin like antibody schwannomin antibody neurofibromin-2 antibody merlin antibody neurofibromin 2 (bilateral acoustic neuroma) antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (3) |
| Cellular Localization | Isoform 1: Cell projection , filopodium membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , ruffle membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 7: Cytoplasm , perinuclear region , Cytoplasmic granule , Isoform |
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Neurofibromin 2 antibody at 1:500 dilution.
Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Neurofibromin 2 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
