Application
Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using MSLN antibody at 1:100 dilution.Application
Sample(30 µg of whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Mesothelin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Mesothelin (mesothelin)An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Mesothelin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MPF antibody, SMRP antibody, MSLN antibody, soluble MPF mesothelin related protein antibody, megakaryocyte potentiating factor antibody, pre-pro-megakaryocyte-potentiating factor antibody, mesothelin antibody, CAK1 antigen antibody |
|---|---|
| Target | Mesothelin |
| Molecular Weight(MW) | 69 kDa (note) (3) |
| Cellular Localization | Cell membrane; Lipid-anchor , GPI-anchor , Golgi apparatus , Megakaryocyte-potentiating factor: Secreted , Isoform 3: Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using MSLN antibody at 1:100 dilution.
Application
Sample(30 µg of whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
