| Product Name | Mesothelin antibody [K1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [K1] to MesothelinThis antibody recognizes CAK 1, a 40 kD cell surface glycoprotein, selectively expressed by malignant mesotheliomas as well as by non-mucinous ovarian carcinomas and squamous tumors of the esophagus and cervix. |
| Immunogen | Spleen lymphocytes from mice fused with OVCAR-3, an ovarian tumorcell line derived from poorly differentiated adenocarcinoma of the ovary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | K1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-F |
| Immunohistochemistry: use at a dilution of 1/40. Optimal dilutions / concentrations should be determined by the end user. Antigen retrieval is not essential, although may be beneficial. In normal adults, this antibody shows intense and uniform reactivity with the mesothelia of the peritoneal, pleural and pericardial cavities, while demonstrating a highly restricted staining pattern in other normal tissues. Strong and homogeneous staining is seen in epithelial-type as well as in mixed-typed malignant mesotheliomas. Lung adenocarcinomas, displaying varying degrees of differentiation, consistently stain negative. The restricted distribution of the CAK1 antigen makes this antibody a potentially versatile reagent for the distinction of epithelial malignant mesothelioma from lung adenocarcinoma in tissue specimens and / or effusion cytological specimens. | |
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | MPF |
|---|---|
| Target | Mesothelin |
| Tissue Specificity | This antibody recognizes CAK 1, a 40 kD cell surface glycoprotein, selectively expressed by malignant mesotheliomas as well as by non-mucinous ovarian carcinomas and squamous tumors of the esophagus and cervix. |
| Cellular Localization | Cell Membrane |
| Positive Control | Tonsil, lymph node and OVCAR-3 cells (human) |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1/40. Optimal dilutions / concentrations should be determined by the end user. Antigen retrieval is not essential, although may be beneficial. In normal adults, this antibody shows intense and uniform reactivity with the mesothelia of the peritoneal, pleural and pericardial cavities, while demonstrating a highly restricted staining pattern in other normal tissues. Strong and homogeneous staining is seen in epithelial-type as well as in mixed-typed malignant mesotheliomas. Lung adenocarcinomas, displaying varying degrees of differentiation, consistently stain negative. The restricted distribution of the CAK1 antigen makes this antibody a potentially versatile reagent for the distinction of epithelial malignant mesothelioma from lung adenocarcinoma in tissue specimens and / or effusion cytological specimens. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS with 1% BSA and 0.1% sodium azide |
